Multiplex Technology for Detection Respiratory tract Infection in Clinical Specimens
نویسندگان
چکیده
منابع مشابه
Respiratory Tract Infection 5. Respiratory Tract Infection
In troduc tion For a better understanding of the pathogenic mechanismof infectious diseases it is important to know the molecular basis of how the host responds to microbial pathogens and eliminates them. Recent advances in immunology and molecular biology have led to the discovery of a large number of cytokines and cell surface molecules, which allows us to understand such pathogenic mechanism...
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BACKGROUND In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea). METHODS Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multipl...
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Respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. The application of nucleic acid amplification technology, particularly multiplex PCR coupled with fluidic or fixed microarrays, provides an important new approach for the detection of multiple respiratory viruses in a single test. The aim of thi...
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The ideal clinical diagnostic system should deliver rapid, sensitive, specific and reproducible results while minimizing the requirements for specialized laboratory facilities and skilled technicians. We describe an integrated diagnostic platform, the "FilmArray", which fully automates the detection and identification of multiple organisms from a single sample in about one hour. An unprocessed ...
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Toll like receptors (TLRs) with a myeloid differentiation antigen (CD14) recognize and bind various structures from invading microbes and then trigger cell activation. They initiate a variety of effectors' functions, including cytokine secretion, proliferation, co-stimulation or phagocyte maturation. The aim of this study was to evaluate Toll-like receptor 4 (TLR4) and CD14 expression in childr...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2008
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2008.05.1306